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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Anal Bioanal Chem. 2014 Jan 31;406(6):1683–1691. doi: 10.1007/s00216-013-7593-7

Fig. 2.

Fig. 2

Flowgram of mitochondria isolated from DsRed2 transfected 143B cells incubated with Atto-488 Anti-TOM22 antibodies. Panel (A) shows examples of mitochondria labeled with both DsRed2 and Atto-488 anti-TOM22 antibodies. Panel (B) shows an expanded view illustrating events observed in a typical flowgram which may include the following: (a) Green fluorescent peaks represent mitochondria events from Atto-488 anti-TOM22 antibody labeling. (b) Red fluorescent peaks represent mitochondrial events from DsRed2 labeling. (c) Simultaneous detection of green and red fluorescent peaks represents mitochondria labeled with both DsRed2 and Atto-488 anti-TOM22 antibodies