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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Nat Med. 2014 Sep 7;20(10):1182–1186. doi: 10.1038/nm.3656

Figure 2.

Figure 2

Stat3 gene deletion enhances satellite cell expansion after skeletal muscle injury. (a) Schematic representation of Tmx treatment in male and female 2-month-old Pax7-CreER; Stat3flox/flox and Pax7-CreER; Stat3+/+ mice. EdU was administered daily for 5 d before harvesting. (b) Stat3, Myod1 and Socs3 mRNA levels in satellite cells isolated from the uninjured skeletal muscle of Tmx-treated Pax7-CreER; Stat3flox/flox and Pax7-CreER; Stat3+/+ mice after culture in growth medium for 96 h (n = 3). (c) Representative images of uninjured skeletal muscle of Tmx-treated Pax7-CreER; Stat3flox/flox and Pax7-CreER; Stat3+/+ mice (green, Pax7; red, EdU; white, laminin; blue, nuclei). Scale bar, 20 µm. (d) Schematic representation of Tmx or vehicle treatment and skeletal muscle injury in male and female 2-month-old Pax7CreER; Stat3flox/flox mice. (e) Left, representative images of Pax7+ satellite cells within the muscles of Tmx- or vehicle (Ctrl)-treated Pax7-CreER; Stat3flox/flox mice 5 d after injury (green, Pax7; white, laminin; blue, nuclei). Scale bar, 50 µm. Right, quantification of Pax7+ satellite cell numbers in regenerating muscles at 5 and 25 d after injury (n = 3). (f) Left, representative images of H&E staining of the muscles of Tmx- or vehicle (Ctrl)-treated Pax7-CreER; Stat3flox/flox mice 25 d after injury. Scale bar, 50 µm. Right, quantification of average myofiber cross-sectional area 25 d after injury (n = 3). All data are represented as the average ± s.e.m. Student’s t test was used for all statistical analyses (***P < 0.001, **P < 0.01, *P < 0.05).