Table 1.
Primers used in this study for qPCR
| Gene | Primer | Sequence (5′-3′) |
|---|---|---|
| Occludin | OccludinF | CAGCCCAGTTCCTCCAGTAG |
| OccludinR | GCTCATCCAGCTCTCTGTCC | |
| Claudin-7 | Claudin-7F | CGTCCTGCTGATTGGATCTC |
| Claudin-7R | CAAACGTACTCCTTGCTGCTG | |
| Claudin-8d | Claudin-8dF | GCAGTGTAAAGTGTACGACTCTCTG |
| Claudin-8dR | CACGAGGAACAGGCATCC | |
| Claudin-12 | Claudin-12F | CTTCATCATCGCCTTCATCTC |
| Claudin-12R | GAGCCAAACAGTAGCCCAGTAG | |
| Claudin-31 | Claudin-31F | TCGGCAACAACATCGTGAC |
| Claudin-31R | CGTCCAGCAGATAGGAACCAG | |
| MUC2 | OmMuc2F | CCAGGCACAGAAAAGACAGATGC |
| OmMuc2R | GGATGTAGGAGTGCTTGACC | |
| MUC5AC | OmMuc5ACF | GCTCTGGTCTTCGGACTATCTG |
| OmMuc5ACR | GCTGCTCTTACACAACGACG | |
| DB-1 | omDB-1F | GGTTTTCCTATTGCTTAATGTTGTGG |
| omDB-1R | GACACACAGTTAAGTCATGG | |
| DB-2 | omDB-2F | GCTGACAGCAGTGCAAGCTGATGACAC |
| omDB-2R | GCAAAGCACAGCATCTTAATCTGC | |
| DB-3 | omDB-3F | GCTTGTGGAATACAAGAGTCATCTGC |
| omDB-3R | GCATACATTCGGCCATGTACATCC | |
| DB-4 | omDB-4F | GCAACTCTTCTAAAGAACAGT |
| omDB-4R | CGTGGGCGACACAGCATACAAATCC | |
| Cathelicidin1 | CATH1F | ACCAGCTCCAAGTCAAGACTTTGAA |
| CATH1R | TGTCCGAATCTTCTGCTGCAA | |
| Cathelicidin2 | CATH2F | ACATGGAGGCAGAAGTTCAGAAGA |
| CATH2R | GAGCCAAACCCAGGACGAGA | |
| Hepcidin | HepcidinF | GCTGTTCCTTTCTCCGAGGTGC |
| HepcidinR | GTGACAGCAGTTGCAGCACCA | |
| LEAP2A | LEAP2AF | GGTTCCTGGTGTTTCTGGTGCT |
| LEAP2AR | AGTGGCCACCCCTGCAAAT | |
| IL1β | IL1bF | ACATTGCCAACCTCATCATCG |
| IL1bR | TTGAGCAGGTCCTTGTCCTTG | |
| TNFα | TNFaF | GGGGACAAACTGTGGACTGA |
| TNFaR | GAAGTTCTTGCCCTGCTCTG | |
| IL6 | IL6F | ACTCCCCTCTGTCACACACC |
| IL6R | GGCAGACAGGTCCTCCACTA | |
| TGFβ1a | TGFb1aF | CTCACATTTTACTGATGTCACTTCCTGT |
| TGFb1aR | GGACAACTGCTCCACCTTGTG | |
| TGFβ1b | TGFb1bF | CATGTCCATCCCCCAGAACT |
| TGFb1bR | GGACAACTGTTCCACCTTGTGTT | |
| EF-1α | EF-1aF | CAACGATATCCGTCGTGGCA |
| EF-1aR | ACAGCGAAACGACCAAGAGG |