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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Neurochem Int. 2014 Mar 19;77:17–23. doi: 10.1016/j.neuint.2014.03.006

Figure 1. OPCs proliferation and OLGs generation in the ischemic white matter.

Figure 1

Panels A–B show quantitative data of NG2+ OPCs (A) and APC+ OLGs (B) in the peri-infarct corpus callosum at 2, 7 and 14 days after MCAO compared to sham control and representative microscopic images of NG2+ OPCs (A) and APC+ OLGs (B) of sham and 7d MCAO groups. n = 6/group. *p<0.05 vs sham group; #p<0.05 vs 2 days group and †p<0.05 vs 7 days group.

Panels C-M show representative microscopic images of double immunofluorescent staining of NG2+ OPCs with BrdU (C to D, arrows) and Ki67 (G to H, arrows) immunoreactivity. Arrowheads in G point at Ki67+ OPCs that appear as doublets. Panels E and I show quantitative data of BrdU (E) and Ki67 (I) immunoreactive OPCs in the ipsilateral and contralateral corpus callosum. Panels K to L show representative microscopic images of APC+ OLGs with BrdU immunoreactivity. Panel M shows quantitative data of BrdU immunoreactive OLGs in the ipsilateral and contralateral corpus callosum. Panels F and J show lack of co-localization of TUNEL+ with BrdU+ cells (F) and APC+ with Ki67+ cells (J) in the peri-infarct corpus callosum. Bar: 20μm. Images (C–M) were taken from the ipsilateral (ischemic) or contralateral (contralateral) corpus callosum. n = 8/group. #p<0.01 and *p<0.05 vs the contralateral corpus callosum.