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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Clin Chem. 2014 Nov 6;61(1):221–230. doi: 10.1373/clinchem.2014.230433

Fig. 5. circRNA expression in CFS.

Fig. 5

Experimental validation of circRNAs in CFS was carried out by RT-PCR. Primers were designed to amplify the circular junctions, as illustrated. CFS samples were collected from the same individuals, but on different days, as for RNA-Seq. PAGE gel images are shown to visualize the presence of the PCR products. Representative Sanger sequencing traces are shown, together with the nucleotide sequences and genomic coordinates (hg19) of the circular junction. All Sanger-based sequences were identical to the predicted circular junctions via RNA-Seq. (A), Canonical circRNAs. Sanger sequencing was conducted on gel-purified PCR products. (B), Noncanonical circRNAs. Sanger sequencing was conducted on random clones amplified after TOPO cloning of the PCR products. DOPEY2, dopey family member 2; UBAP2, ubiquitin associated protein 2; GSE1, Gse1 coiled-coil protein; ASXL1, additional sex combs like transcriptional regulator 1; UGP2, UDP-glucose pyrophosphorylase 2; WDFY1, WD repeat and FYVE domain containing 1. D1, M1, S1, and C1 are study-assigned IDs for samples. M, marker.