Table 2.
Fermentation products (mmol l −1 ) of hyper-ammonia-producing bacterial isolates* obtained from Nellore steers
| Concentration of fermentation end-products | Isolate ID | Total VFA | A | P | B | Ib | F | S | Iv |
|---|---|---|---|---|---|---|---|---|---|
| (mmol l −1 **) | (%) | ||||||||
| High | C48 | 79.9 ± 2.35a | 47.8 | 8.1 | 7.8 | 5.3 | 4.37 | 4.8 | 21.5 |
| C51 | 76.6 ± 1.74a | 54.2 | 7.1 | 7.3 | 5.1 | 1.57 | 1.5 | 23.0 | |
| C33 | 73.3 ± 3.16a | 55.4 | 7.9 | 5.8 | 5.9 | 2.37 | nd | 22.3 | |
| R34 | 71.0 ± 1.30a | 37.4 | 3.7 | 5.9 | 5.3 | 1.78 | 1.7 | 44.0 | |
| C11 | 68.4 ± 4.84a | 69.1 | 11.8 | 6.2 | 7.2 | 1.32 | nd | 4.2 | |
| R36 | 66.7 ± 3.14a | 50.3 | 11.3 | 5.1 | 5.1 | 1.48 | nd | 26.5 | |
| C47 | 64.4 ± 1.94a | 49.2 | 8.4 | 7.7 | 5.8 | 1.95 | nd | 26.7 | |
| C37 | 62.5 ± 0.99a | 65.9 | 13.9 | 6.8 | 6.5 | 1.69 | nd | 5.0 | |
| R40 | 60.6 ± 5.15a | 37.8 | 10.2 | 5.5 | 4.0 | 9.48 | nd | 32.9 | |
| R90 | 60.2 ± 2.34a | 64.9 | 9.7 | 5.4 | 9.7 | 3.63 | 1.5 | 4.9 | |
| R15 | 59.6 ± 2.90a | 67.4 | 10.1 | 6.9 | 8.2 | 1.94 | 0.5 | 4.7 | |
| R23 | 53.7 ± 3.47a | 61.8 | 12.0 | 8.3 | 9.5 | 5.14 | nd | 3.0 | |
| Medium | C89 | 49.2 ± 1.69b | 57.1 | 9.5 | 7.3 | 6.0 | 14.9 | 2.5 | 2.4 |
| C34 | 45.5 ± 3.25b | 63.3 | 14.6 | 7.2 | 7.2 | 3.00 | nd | 4.5 | |
| C122 | 45.0 ± 2.42b | 57.9 | 10.4 | 7.3 | 5.6 | 13.44 | 2.9 | 2.2 | |
| R50 | 44.2 ± 2.09b | 66.8 | 10.1 | 7.3 | 7.8 | 2.89 | nd | 4.9 | |
| R61 | 44.0 ± 3.20b | 39.0 | 7.3 | 6.6 | 4.4 | nd | nd | 42.5 | |
| C114 | 43.6 ± 1.05b | 51.4 | 10.4 | 6.5 | 7.8 | 21.72 | nd | 2.0 | |
| C118 | 41.6 ± 1.21b | 55.7 | 10.1 | 7.4 | 7.9 | 12.63 | 3.2 | 2.6 | |
| R51 | 39.0 ± 3.43b | 50.1 | 16.9 | 9.0 | 6.8 | 13.43 | 1.6 | 1.9 | |
| C54 | 38.3 ± 1.88b | 51.5 | 16.5 | 8.8 | 8.3 | 12.86 | nd | 1.8 | |
| C117 | 34.5 ± 3.92b | 49.4 | 15.0 | 2.6 | 13.6 | 8.69 | 6.3 | 4.1 | |
| Low | R21 | 30.6 ± 6.17c | 71.5 | 9.0 | 8.7 | 5.9 | nd | nd | 4.7 |
| R60 | 30.3 ± 2.56c | 66.0 | 8.4 | 13.5 | 6.2 | nd | nd | 5.8 | |
| R91 | 25.0 ± 1.05c | 20.2 | 10.2 | 3.5 | 56.2 | 8.02 | nd | 1.6 | |
| R63 | 22.9 ± 0.61c | 64.7 | 11.6 | 10.3 | 7.3 | nd | nd | 5.9 | |
| R107 | 21.5 ± 2.11c | 58.8 | 15.4 | 3.6 | 13.5 | 6.59 | nd | 1.9 | |
| C116 | 19.3 ± 4.17c | 50.0 | 17.2 | nd | 15.4 | 10.61 | 6.6 | nd | |
| R96 | 16.9 ± 3.34c | 52.2 | 17.3 | 4.5 | 11.6 | 11.13 | nd | 3.0 | |
| R97 | 12.2 ± 5.97c | 47.8 | 25.7 | nd | 7.3 | 19.05 | nd | nd |
A = acetate; P = propionate; B = butyrate; Ib = isobutyrate; F = formate; S = succinate; Iv = isovalerate; nd = not detected.
Values are provided as the mean ± standard deviation of the mean.
*The isolates were grown in anaerobic mineral medium supplemented with 15 g l−1 trypticase for 24 h at 39°C.
**Averages of total volatile fatty acids followed by different letters in the same column differ at 5% probability by the Scott-Knott test.