Table 4.

Proteolytic and ureolytic activities of hyper-ammonia-producing bacterial isolates* obtained from Nellore steers

Isolate ID**	Proteolytic activity in trypticase 1	Proteolytic activity in Casein 1	Ureolytic activity in trypticase + urea 2
C11	0.12 ± 0.01	0.86 ± 0.06	1.74 ± 0.01
C33	0.01 ± 0.01	1.18 ± 0.04	0.80 ± 0.01
C34	0.41 ± 0.01	10.87 ± 0.08	2.37 ± 0.05
C37	0.07 ± 0.01	0.40 ± 0.03	6.03 ± 0.15
C47	0.16 ± 0.01	0.89 ± 0.08	12.07 ± 0.06
C48	1.19 ± 0.03	4.30 ± 0.16	0.78 ± 0.01
C51	0.09 ± 0.01	0.18 ± 0.01	1.96 ± 0.03
C54	1.61 ± 0.01	5.17 ± 0.26	3.46 ± 0.04
C89	0.13 ± 0.01	2.52 ± 0.02	6.01 ± 0.10
C114	0.17 ± 0.01	0.66 ± 0.01	1.50 ± 0.03
C116	0.14 ± 0.01	0.83 ± 0.07	3.10 ± 0.01
C117	1.25 ± 0.01	31.26 ± 1.76	15.55 ± 0.39
C118	0.13 ± 0.01	0.91 ± 0.01	4.81 ± 0.03
C122	0.70 ± 0.01	1.36 ± 0.09	2.33 ± 0.02
R15	0.11 ± 0.01	0.38 ± 0.01	2.06 ± 0.08
R21	0.12 ± 0.01	0.81 ± 0.04	1.05 ± 0.01
R23	0.09 ± 0.01	4.02 ± 0.16	1.81 ± 0.03
R34	0.34 ± 0.02	0.91 ± 0.08	1.28 ± 0.01
R36	0.91 ± 0.01	1.55 ± 0.09	3.77 ± 0.08
R40	2.46 ± 0.02	9.48 ± 0.37	7.44 ± 0.04
R50	0.78 ± 0.03	1.65 ± 0.17	2.67 ± 0.03
R51	0.85 ± 0.01	0.87 ± 0.04	1.41 ± 0.02
R60	0.08 ± 0.01	0.61 ± 0.06	1.36 ± 0.02
R61	0.10 ± 0.01	0.42 ± 0.04	1.33 ± 0.02
R63	0.13 ± 0.01	0.46 ± 0.07	1.11 ± 0.01
R90	0.22 ± 0.01	0.79 ± 0.07	2.41 ± 0.03
R91	0.22 ± 0.01	1.38 ± 0.38	2.07 ± 0.04
R96	0.19 ± 0.01	0.68 ± 0.09	1.55 ± 0.03
R97	0.05 ± 0.01	1.17 ± 0.13	4.00 ± 0.06
R107	0.12 ± 0.01	0.99 ± 0.31	10.37 ± 0.41

*Cultures were incubated for 48 h at 39°C in anaerobic mineral medium with limited nitrogen before the enzymatic activities were determined.

**C, isolates from animals not supplemented with casein; R, isolates obtained from animals that received ruminal infusion of casein.

¹U μg protein⁻¹.

²μmol NH₃ mg protein⁻¹ min⁻¹.

Values are given as mean ± standard deviation of the mean.