Figure 2. Mutant transgenic mice have severe skeletal defects and perinatal lethality.

Transgenic mice overexpressing the mutant Ifitm5 show limb deformities compared to non-transgenic littermates and wild type transgenics at E18.5 (A). Radiographs of E18.5 mutant transgenics show reduced mineralization, while wild type transgenics appear normal (B). Skeletal preparations of mutant transgenics at E15.5 (C) and E18.5 (D) show reduced alizarin red staining and fractures which are not evident in wild type transgenic or non-transgenic littermates. Skeletal preparations of E18.5 mutant transgenic mice compared to wild type transgenic controls show abnormalities of the forelimbs, hindlimbs and ribs (E).