Figure 3. Mutant transgenic mice have in utero fractures and morphological defects of the hindlimbs.

Histology of the E15.5 tibia of non-transgenic controls and mutant transgenic mice stained with H&E with arrow indicating limb dysplasia (A), and with alcian blue and nuclear fast red with arrow indicating growth plate expansion (B). Histology of E18.5 non-transgenic controls, mutant transgenic littermates, and wild-type transgenic control mice by H&E, picrosirius red, alcian blue with nuclear fast red, and von kossa staining of the hindlimbs (C). The growth plate is extended in mutant transgenics, as indicated by alcian blue staining of the proximal tibia (D). Bone morphology is altered in the proximal tibia, as shown in picrosirius red staining (E).