Figure 4. Calvarial osteoblasts from mutant transgenics have mineralization defects.

Alkaline phosphatase staining of mutant and wild type transgenic calvarial osteoblasts shows no changes (A). Alizarin red staining of calvarial osteoblasts shows a mineralization defect in mutant transgenics but not in wild type transgenics as compared to non-transgenic littermates (n=5-6 biological replicates plus 3 technical replicates of each) (B). Quantitative real-time PCR shows no change in Runx2 levels in calvarial bone samples, but a reduction in Sp7, Col1a1, Bsp and Ocn levels (C). Results from a t-test in (A, B, and C) are means ± SD, *p < .05.