Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell Signal. 2015 Jan 10;27(3):487–497. doi: 10.1016/j.cellsig.2015.01.004

Fig. 3.

Fig. 3

Gli2 and Gli3 are involved in Hh-induced conversion of cholesterol in JEG-3 cells. (A–C) P450scc, 3β-HSD1 and aromatase expression in JEG-3 cells, after infection with Gli1-, Gli2- or Gli3-shRNA-expressing lentivirus and treatment with either control medium (CM) or Shh conditional medium (SM) for 48 hrs. Quantification via densitometry (n=3) and statistical analysis of bands were also performed. (D–F) P450scc, 3β-HSD1 and aromatase expression in JEG-3 cells, after infection with either constitutively active form of Gli2- (ΔN-Gli2) or Gli3 (Gli3-ΔC)-expressing lentivirus for 48 h. (G, H) P4 and E2 levels in JEG-3 cells, after infection with Gli1-, Gli2- or Gli3-shRNA-expressing lentivirus and treatment with either control medium (CM) or Shh conditional medium (SM) for 48 hrs. RNA and protein abundance normalized to β-actin, respectively. **, ‡p<0.01, † p<0.05; n=3, error bar, SD.