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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell Signal. 2015 Jan 10;27(3):487–497. doi: 10.1016/j.cellsig.2015.01.004

Fig. 4.

Fig. 4

Hh transactivates P450scc, 3β-HSD1 and aromatase in Gli response elements-dependent manners in JEG-3 cells. (A–C) Schematic presentation of 5′-untranscriptional region and the potential Gli binding sites on P450scc, 3β-HSD1 and aromatase promoters. (D–F) Dual-luciferase assays in JEG-3 cells, after transient transfection with reporter constructs of wild type or deletion mutants for P450scc, 3β-HSD1 and aromatase, and treatment with either control medium (CM) or Shh conditional medium (SM) for 48 h. (G–I) Dual-luciferase assays in JEG-3 cells, after transient transfection with reporter constructs of wild type or Gli binding site mutants for P450scc, 3β-HSD1 and aromatase. **, ‡p<0.01, *,† p<0.05; n=6, error bar, SD.