MicroCT analysis of femur midshaft cortical bone showed that total area (T.Ar, A), bone area (B.Ar, B) and MMI (C) were significantly increased in Δ130-136 mice in comparison to WT and R76W mice. Data shown are mean ± SD. **, P < 0.01; ***, P < 0.001. n = 11-17. Three-point bending assay was performed on isolated femur bones of 4-month old, male WT and transgenic mice, and stiffness (D), yield force (E), elastic modulus (F) and ultimate tensile strength (G) were measured. Yield force (E) was significantly greater in Δ130-136 mice compared to WT and R76W mice. A significant reduction was observed in the elastic modulus (F) and ultimate tensile strength (G) of Δ130-136 compared to WT and R76W mice. Data shown are mean ± SD. *, P < 0.05; **, P < 0.01. n = 6.