FIG 5.

Whole-mount immunohistochemistry with the HCS-1 antiotoferlin antibody. (A to D) Images of showing otoferlin expression in 120-hpf larvae as follows: control injected (A), otoferlin b KD (B), otoferlin a KD (C), and otoferlin b+a KD (D). (E to F) Fluorescent maximum-intensity projection image of 120-hpf larval otic region of injected control (E), otoferlin b KD (F), and otoferlin a KD (G) larvae showing distinct distribution of otoferlin in the sensory patches of cristae and maculae. ac, anterior crista; mc, medial crista; pc, posterior crista; pm, posterior macula; am, anterior macula.