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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell Signal. 2014 Nov 25;27(3):707–715. doi: 10.1016/j.cellsig.2014.11.018

Figure 6. Insulin inhibits βAR-induced contractile shortening in neonatal and adult mouse cardiomyocytes.

Figure 6

Wild type neonatal cardiomyocytes were stimulated with ISO in the presence and absence of pretreatment with insulin (100 nM, 30 min) as indicated (A and B). The changes in spontaneous contraction rates were recorded. (A) Traces show time course of increases in contraction rates over ISO stimulation in representative dishes. The ISO dose-dependent increases (B) and the maximal increases (C) of contraction rates were plotted. # p < 0.05 by two-way ANOVA and * p < 0.05 by one-way ANOVA followed by Tukey’s test between groups. (D) Adult mouse cardiomyocytes paced at 1 Hz were pretreated with insulin (100 nM, 30 min) before stimulation with ISO (100 nM). Contractile shortening was recorded and the maximal increases of contraction rates (E) were plotted. * p < 0.05 by one-way ANOVA followed by Tukey’s test between groups; n = 6~7 from three independent experiments.