Figure 2. Detailed timelines of (A) the study protocol and (B) the flow-mediated dilation assessment.

(A) The effect on brachial artery flow-mediated dilation after glucose loading was assessed repeatedly, sessions 1 and 2 about one week apart. (B) A 5-minute lower arm cuff-occlusion was used to elicit a hyperemic response to measure flow-mediated dilation from a continuous brachial Duplex ultrasound recording (B-mode and pulsed Doppler). Note the stimulus-response type of assessments for both (A) and (B) and the correspondingly different timescales.