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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: J Gerontol A Biol Sci Med Sci. 2008 Mar;63(3):242–252. doi: 10.1093/gerona/63.3.242

Figure 2.

Figure 2

Mean adult life span of wild-type (N2) Caenorhabditis elegans hermaphrodites at 22.5°C on different concentrations of axenic medium (AXM) in agar plates. Standard AXM (100%) was diluted to 50% and 10%, and concentrated to 200% and 300%. *Indicates statistical significant changes as compared to 100% AXM (mean adult life span on 100% AXM = 25.0 ± 0.3 days, N = 156 (dead)/44 (censored); on 10% AXM = 23.3 ± 0.7 days, N = 35/165; on 50% AXM = 22.2 ± 0.3 days, N = 187/35; on 200% AXM = 26.9 ± 0.5 days, N = 90/70; on 300% AXM = 13.8 ± 1.1 days, N = 29/121) (p < .0001 for all comparisons). Between 10% and 200% AXM life span was statistically significantly, but only marginally affected by the medium concentration. Carbenicillin-treated Escherichia coli OP50 was added to 7.5 × 108 cells/plate to a separate series of AXM concentrations to control for toxic effects of the medium. **Indicates statistically significant changes as compared to 100% AXM with E. coli (mean adult life span on 100% AXM + E. coli = 19.8 ± 0.2 days, N = 204/16; on 10% AXM + E. coli = 17.6 ± 0.2 days, N = 128/26; on 50% AXM + E. coli = 19.6 ± 0.2 days, N = 133/10; on 200% AXM + E. coli = 15.5 ± 0.3 days, N = 172/25; on 300% AXM + E. coli = 10.0 ± 0.3 days, N = 62/38) (p < .0001 for all comparisons).