Fig. 3.

The effect of HMGB1 on proliferation, migration, and cytokine production by TLR4-silenced HaCaT cells. Cell proliferation and migration assays were performed after 72 h of culture using the xCELLigence System, and IL-8 level was measured by ELISA assay, as described in “Materials and methods.” HMGB1 (100 ng/mL) induced significant proliferation, migration, and IL-8 production in HaCaT cells. Antibodies specific for RAGE almost completely blocked the effects mediated by HMGB1. The data are from six experiments with each sample run in triplicate, and the p values confirm significance of the receptor blockade