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. 2014 Aug 3;11(1):110–118. doi: 10.1007/s12015-014-9547-7

Fig. 2.

Fig. 2

HO-1 deficiency reduces adhesive potential of bone marrow stromal cells. Panel a - Bone marrow mononuclear cells (BMMNC) and BM stromal cells were derived from bone marrow of HO-1+/+ (WT), HO-1+/− and HO-1−/− mice. Total RNA was isolated and quantitative real-time PCR was performed to determine expression of CXCR4 in BMMNC and SDF-1 and beta defensine-2 expression in BM stroma cells. * p < 0.05. Panel b – ELISA results demonstrating decrease in SDF-1 level in conditioned media harvested from BM stroma cell cultures from HO-1 deficient animals. * p < 0.05. Panel c - HO-1 does not affect adhesion of CFU-GMs to stromal cells isolated from wild type mice (left), however wild type CFU-GM have impaired adhesion to stromal cells isolated from HO-1+/− and HO-1−/− mice in comparison to HO-1+/+ (WT) (right). * p < 0.05. Number of CFU-GM colonies that adhered to stroma cells in left and right panel were assumed to be 100 %. Data represent average of three independent experiments (a–c)