Fig. 4.

P2Y1 mediated neuronal chain migration induced by a physiological EF. a Western blotting showed the P2Y1 band at 45KD to be up-regulated in a time dependent manner by a physiological EF, with an increase evident within 1 h. b Neurospheres from mouse SVZ were seeded in electrotaxis chambers with/without an applied EF for 5 h and then cells were fixed and stained immunofluorescently with P2Y1 receptor antibody. The applied EF significantly increased the expression of P2Y1 in the cells which migrated out from the cultured mouse neurosphere. c Inhibition of P2Y1 receptors with a specific inhibitor (100 μM MRS2179) significantly reduced the expression of N-cadherin and β-catenin and blocked the activation of PKC using western blotting and mouse neurospheres. d Inhibition of P2Y1 receptors with siRNA also effectively inhibited the expression of N-cadherin and activation of PKC. Graphs below C and D demonstrate corresponding quantification. All experiments were triplicate