FIG. 2.
Characterization of hepatic injury mouse model. (A) 20% carbon tetrachloride (CCl4) was injected into mice every 3 days for 30 days to induce chronic liver damage. Microscopic pictures were taken from livers of normal mice (Control) and liver-injured mice (CCl4). The difference of livers from normal and liver-injured mice is very obvious. (B) Blood samples were collected from normal and liver-injured mice. Concentrations of serum alanine aminotransferase (ALT), total bilirubin (TB), and albumin (ALB) were measured. The results showed that ALT and TB were significantly higher, and ALB was significantly lower after 14 and 30 days of CCl4 injection. *p<0.01 versus respective normal mice control. (C) Livers were removed from normal and injured mice after 14 and 30 days of CCl4 injection. Liver tissue sections were examined after hematoxylin-eosin (HE) and Masson staining. Liver damage was observed and liver showed blue by Masson staining for collagen deposition after CCl4 injection. Color images available online at www.liebertpub.com/tea