FIG. 4.

Corticosterone inhibits MSCs migration in vitro. (A) RT-PCR was performed to detect the expression of CR mRNA in cultured MSCs and BM derived from mice, and the amplified products were visualized in the agarose gel incorporated with 0.5 g/mL of ethidium bromide. (B) MSCs were cultured in DMEM-low glucose with 10% mice serum of indicated groups with or without 1×10⁻⁷ M corticosterone or 1×10⁻⁷ M RU486 for 48 h, and then they were subjected to transwell migration assay. Representative photographs of the migrated MSCs are shown. (C) Quantification of three independent experiments of transwell migration assay is shown. This is the significance of the quantification of violet cells. (**P<0.01 between the indicated groups; ×200 magnification). BM, bone marrow; CORT, corticosterone; DMEM, Dulbecco's modified Eagle's medium. Color images available online at www.liebertpub.com/scd