Table 1. Oligonucleotide primers used in this study.
Gene | Method | Forward sequence | Reverse sequence |
---|---|---|---|
GPR158 | RT-PCR | atattgctacagaagcatatgag | atatttccagttgggcatagag |
AR | RT-PCR | caggaaagcgacttcaccgc | ctggggtggaaagtaatagtc |
PSA | RT-PCR | ggcagcattgaaccagaggag | gcatgaacttggtcaccttctg |
NSE | RT-PCR | ctcatcagctcaggtctctc | ccttacacacggccagagac |
β-actin | RT-PCR | cattgccgacaggatgcaga | ctgatccacatctgctggaa |
GAPDH | RT-PCR | aacctgccaagtacgatgacatc | gtagcccaggatgcccttga |
GPR158:ΔC | PCR | Ttaattgctagcatgggagccatggcttacc | gcttaccggtggtggaatcaaaagcaacccaatggtgac |
The abbreviations are: GPR158, G protein-coupled receptor 158; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction; RT-PCR, reverse transcriptase–polymerase chain reaction.