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. 2015 Feb 18;10(2):e0117758. doi: 10.1371/journal.pone.0117758

Table 1. Oligonucleotide primers used in this study.

Gene Method Forward sequence Reverse sequence
GPR158 RT-PCR atattgctacagaagcatatgag atatttccagttgggcatagag
AR RT-PCR caggaaagcgacttcaccgc ctggggtggaaagtaatagtc
PSA RT-PCR ggcagcattgaaccagaggag gcatgaacttggtcaccttctg
NSE RT-PCR ctcatcagctcaggtctctc ccttacacacggccagagac
β-actin RT-PCR cattgccgacaggatgcaga ctgatccacatctgctggaa
GAPDH RT-PCR aacctgccaagtacgatgacatc gtagcccaggatgcccttga
GPR158:ΔC PCR Ttaattgctagcatgggagccatggcttacc gcttaccggtggtggaatcaaaagcaacccaatggtgac

The abbreviations are: GPR158, G protein-coupled receptor 158; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction; RT-PCR, reverse transcriptase–polymerase chain reaction.