Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 5;43(2):e8. doi: 10.1093/nar/gku1087

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — Identification of natural AAs and Sep incorporated at native UAG codons in Escherichia coli. (A) Summary of peptides spanning UAG sites from RF1 + BL21 strains (BL21.WT.SEP.OTS2.1 (green) and BL21.L11C.SEP.OTS2.1 (violet)) and the RF1 deficient strain EcAR7.SEP.OTS2.1 (brown) discovered with the proteomics workflow. Peptides with Sep@UAG (red), Q@UAG (white or red underlined; *two Q in sucB) and V@UAG (black, italic) are listed. (B) Annotated tandem MS spectrum of a phosphopeptide showing incorporation of Sep at the native UAG codon in speG.