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. 2014 Nov 6;43(2):e9. doi: 10.1093/nar/gku1090

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Pre-let-7a-2 is cleaved by Dicer into mature miRNAs in the presence of let-7 looptomirs. (a) Schematic cleavage of pre-miRNAs by Dicer into mature miRNA 5p and 3p strands. (b) In vitro-transcribed pre-let-7a-2 incubated with recombinant Dicer for 150 min: reaction mixtures were analyzed by RP-HPLC, and peaks were identified by MS (inset: HPLC-chromatogram of pre-let-7a-2 used in the study). Pre-let-7a-2 incubated under identical conditions with recombinant Dicer and Lcon (c) and L29-13 (d). Products were analyzed by RP-HPLC. (e) Masses observed and calculated for expected miRNA products from (b).