Figure 2.

The ParB–parS complex does not recruit additional ParB molecules to neighbouring non-specific DNA. The binding of ParB to a 147-bp DNA labelled with Cy3 (Supplementary Table S1) results in an increase in fluorescence intensity. (A) Titration of ParB dimer on DNA containing a parS site in its centre. (B) Titration on an equivalent substrate that is lacking the parS site. These data were fit to Equation (1) to yield the values shown. The error bars represent the standard errors from three independent experiments.