Figure 2.

Recruitment of OGG1 and XRCC1 under different laser conditions. Recruitment kinetics of (A) the BER protein OGG1, (B) BER/SSBR scaffold protein XRCC1 and (C) XRCC1 mutant L360D were compared following irradiation of HeLa cells expressing pEGFP-OGG1 or wild type or mutant XRCC1-mGFP with either 750-nm multi-photon excitation or 405-nm laser excitation. The recruitment of the XRCC1 mutant, L360D, was tested in cells co-expressing WT XRCC1-mRFP (shown in Supplementary Figure S2). Error bars represent SEM from three independent experiments each analyzing 12 cells (i.e. n = 36).