Figure 5.

PARP1 inhibition and the recruitment of SSBR proteins to sites of DNA damage. HeLa cells expressing fluorescently tagged SSBR proteins were treated with the PARP inhibitor, AG14361, and then subjected to laser micro-irradiation. PARP inhibition only affected early recruitment events of (A) XRCC1, (B) PNKP and (C) LIG3 with almost no effect on the late events of accumulation of all the proteins at sites of DNA damage. For recruitment curves, error bars represent SEM from three independent experiments for a total of 36 individual cells.