Figure 7.

Comparison between the recruitment of full-length LIG3 and LIG3 lacking the zinc finger to sites of DNA damage. (A) HeLa cells or (B) EM9 cells expressing full-length (FL) LIG3 or mutant ΔZnF LIG3 were subjected to laser micro-irradiation. In HeLa cells, FL LIG3 was robustly recruited to sites of DNA damage while ΔZnF LIG3 was recruited less efficiently. Furthermore, FL-LIG3 was recruited to sites of DNA damage even in the absence of XRCC1 (EM9 cells) while ΔZnF-LIG3 could not. For recruitment curves, error bars represent SEM; n = 36. Both cell lines were tested with mRFP- and EGFP-tagged proteins and the tags were shown not to influence the result.