Table 2. Individual changes introduced into the lacZα indicator gene of pSJ4 by human Prim-Pol are shown.
| Mutation Type | Number | Frequency (%) |
|---|---|---|
| A→T/T→A | 1 | 1.05 |
| A→C/T→G | 2 | 2.1 |
| A→G/T→C | 0 | 0 |
| G→A/C→T | 24 | 25.3 |
| G→C/C→G | 4 | 4.2 |
| G→T/C→A | 2 | 2.1 |
| Insertions | 20 | |
| Deletions | 42 | 44.2 |
| Total | 95 | 100 |
| A→N/T→N | 3 | 3.16 |
| G→N/C→N | 30 | 31.6 |
| Transitions | 24 | 25.3 |
| Transversions | 9 | 9.5 |
A total of 95 white colonies were completely sequenced in order to reveal the actual types and frequency of mutations.