Table 3. PrimPol error rates within STR and HSV-tk coding sequences.
| Mutational Target | Frequency x 10−4 | |
|---|---|---|
| T8 | A8 | |
| Observed HSV-tk frequency ± SDa | 1400 ± 360 | 900 ± 210 |
| ssDNA Background | 0.7 | 0.7 |
| Pol EFestb | 1300 (59)c | 770 (40) |
| STR Region | 470 (21) | 210 (11) |
| HSV-tk Coding Region | 860 (38) | 560 (29) |
| Frameshifts | 590 (26) | 380 (20) |
| Large Deletions | 110 (5) | 100 (5) |
| Complex | 160 (7) | 80 (4) |
| Base Substitutions | <23 (0) | <19 (0) |
aMutant frequencies are mean ± standard deviation of three independent reactions.
bPol EFest is calculated as described in methods.
cNumber of independent errors from two reactions.