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. 2014 Dec 24;43(2):745–759. doi: 10.1093/nar/gku1324

Figure 7.

Figure 7.

The Kcnq1ot1 ncRNA is spliced. (A) Long RNA-sequencing data showing strand-specific expression from ENCODE, visualized in the UCSC genome browser. Minus strand codes for the Kcnq1ot1 lncRNA; the plus strand codes for Kcnq1. Higher Kcnq1 expression correlates with lower and more discontinuous Kcnq1ot1 expression. (B) Confirmation of predicted splicing events for the Kcnq1ot1 lncRNA. Top, schematic of the Kcnq1ot coding region and the splicing variants (not to scale). cDNA from neonatal heart was used as substrates for qPCR. Shown is a 2% agarose gel with PCR products from neonatal heart. Primer sets 1F-2R and 2F-3R are designed to detect the junctions between exon 1 and 2 (190 bp), and 2 and 3 (369 bp), respectively. Primers 1F-i1R detect the unspliced version (297 bp). Exons 1 and 2 are 247 and 1043 bp, respectively.