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. 2015 Jan 6;43(2):1069–1080. doi: 10.1093/nar/gku1328

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — EWS-FLI1 binds to RNA. (A) Concentrations from 0.5 to 64 nM of EWS-FLI1 bind to ³²P-labeled 350-nt long dsRNA in gel shift assay, representative of three experiments. (B) Concentrations from 0.5 to 64 nM of RHA bind to ³²P-labeled 350-nt long dsRNA in gel shift assay, representative of three experiments. (C) EWS-FLI1 binds to dsRNA. i–ii: AFM images of RNA-bound EWS-FLI1. The green object is dsRNA and the pink object is EWS-FLI1 protein. The color scale given on the right side of the image represents the height of the protein on the surface. (D) The volume distribution of RNA bound EWS-FLI1 (as the calculated volume of EWS-FLI1 was 104 nm³) protein complexes. The number of black spheres on the bar graphs represents the number of EWS-FLI1 protein on RNA complexes. (E) RHA binds to RNA. i–ii: AFM images of RNA-bound RHA. The green object is dsRNA and the pink object is RHA protein. (F) The volume distribution of RNA-bound RHA (as the calculated volume of EWS-FLI1 was 267 nm³) protein complexes. The number of gray spheres on the bar graphs represents the number of RHA proteins on RNA complexes.