Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 6;43(2):1069–1080. doi: 10.1093/nar/gku1328

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — YK-4-279 negates the inhibitory effect of EWS-FLI1 on RHA in an enantiomer-specific manner and alters the profile of bound RNA. (A) Recombinant RHA (1 nM) in the presence of recombinant EWS-FLI1 (1 nM) or small molecules (30 μM). Helicase reactions carried out as shown above in Figure 1. (B) Triplicate results of helicase reactions were plotted over time. The x-axis is time in seconds and the y-axis is percent product formation in the helicase assay. (C) Scheme used for RNA immunoprecipitation (RIP) of RHA or EWS-FLI1 protein complexes. TC32 ES cells either treated with DMSO or 3-μM YK-4-279 for 8 h. Extraction of RNA associated with RHA or EWS-FLI1 complexes was followed by Illumina sequencing. (D) 1345 transcripts were found common in RHA or EWS-FLI1 RIP. YK-4-279 changes the profile of 1345 RNA bound to the proteins. i: after YK-4-279 treatment, 615 of 1345 RNAs were immunoprecipitated separately by either RHA or EWS-FLI1 antibodies. ii: 306 transcripts were immunoprecipitated by EWS-FLI1. iii: RHA RIP yielded 243 transcripts. iv: 181 transcripts were not found in sequencing from either IP reaction.