Table 1. Lengths and sequences of oligonucleotide primers used for site-directed mutagenesis and DNA amplification.
| Oligonucleotide | Length (b) | 5′ → 3′ Sequence |
|---|---|---|
| 1 | 39 | ACCTGCCAGCTTCCCATTAACCGGGGTGGAGGTGAAGGA |
| 2 | 39 | TCCTTCACCTCCACCCCGGTTAATGGGAAGCTGGCAGGT |
| 3 | 38 | GCCAGCTTCCCATTGACCAGGGTGGAGGTGAAGGAAAG |
| 4 | 38 | CTTTCCTTCACCTCCACCCTGGTCAATGGGAAGCTGGC |
| 5 | 39 | CAGCTTCCCATTGACCGGGATGGAGGTGAAGGAAAGGCC |
| 6 | 39 | GGCCTTTCCTTCACCTCCATCCCGGTCAATGGGAAGCTG |
| 7 | 22 | CCAGTTCTGACAGGACTAGCTG |
| 8 | 23 | GGACGTGGGGTTATAGAAGAGAC |
| 9 | 21 | GTCATGAGGAGCTTGGTCAGC |
| 10 | 21 | GCCAAACTAACCCTGGCAATC |
Oligonucleotides 1–6 were used for QuikChange site-directed mutagenesis of human RAD51. Underlined trinucleotide sequences represent codon changes with respect to wild-type RAD51. Oligonucleotides 7–10 were used for nested PCR to amplify and sequence exon 6 of the RAD51 gene from breast tumors. See ‘Materials and Methods’ section for details.