Table 3. Percentage of time that a hydrogen bond is present in water and methanol simulations at the window around the minimum of the PMF as determined by the restraining distance R₀.

	ARG		LYSH		THR		SER		TYR		ASN		GLN		ASP		GLU
	wat	met	wat	met	wat	met	wat	met	wat	met	wat	met	wat	met	wat	met	wat	met
GUA-N1	0	0	0	0	2	11	4	11	0	4	1	8	1	9	54	92	53	77
GUA-N2	0	0	0	0	3	9	4	10	0	4	3	11	2	11	32	79	33	88
GUA-O6	0	32	8	30	2	6	3	7	0	1	2	13	1	11	0	0	0	0
GUA-N7	0	28	7	24	2	4	2	3	0	1	3	10	2	4	0	0	0	0
CYT-O2	3	59	11	36	4	14	7	13	0	3	4	16	4	16	0	0	0	0
CYT-N3	2	35	5	17	3	6	3	1	0	1	5	17	3	17	0	0	0	0
CYT-N4	0	0	0	0	5	10	4	12	0	7	4	22	4	16	37	76	36	80
URA-N3	0	0	0	0	5	13	5	14	0	0	1	7	4	9	45	31	33	26
THY-N3	0	0	0	0	6	9	8	19	0	0	0	4	3	9	44	62	28	68

We only show hydrogen bonds that were present more than 20% of the time.