Table 1. Dissociation constants for hADAR2-D binding to 8-azaN RNAs.
RNA | Kd (nM)a |
---|---|
(b) | 27 ± 16 |
(c) | 310 ± 60 |
(d) | 30 ± 8 |
(e) | >3200 |
(f) | 114 ± 11 |
(g) | 99 ± 37 |
(h) | 342 ± 88 |
(i) | 15 ± 2 |
(j) | 10 ± 2 |
aSamples were equilibrated in 20-mM Tris-HCL, pH 7.1, 6% glycerol, 0.5-mM DTT, 60-mM KCl, 20-mM NaCl, 0.1-mM 2-mercaptoethanol, 1.5-mM EDTA, 0.003% NP-40, 160-units/ml RNasin, 100-μg/ml BSA and 1.0-μg/ml yeast tRNA for 30 min at 30°C.