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. 2014 Dec 24;43(2):775–786. doi: 10.1093/nar/gku1346

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — GFP-H3.1 A31S overexpression does not inhibit myogenesis and GFP-H3.3 S31A overexpression does not enhance myogenesis. (A) Q-PCR. mRNA expression levels of SKM marker genes were repressed in C2C12 cells expressing GFP-H3.1, whereas they were not in those expressing GFP-H3.1 A31S. Relative values to differentiated WT cells are presented (mean ± standard deviation of three independent experiments). (B) Immunofluorescence with Myog. (C) Box plot representation of the Myog-positive fraction among total nuclei identified by Hoechst staining. Ten different fields from images like (B) were analyzed. Scale bar, 20 μm. (D) Q-PCR. mRNA expression levels of SKM marker genes were enhanced in C2C12 cells expressing GFP-H3.3, whereas it was not in those expressing GFP-H3.3 S31A. Relative values to differentiated WT cells are presented (mean ± standard deviation of three independent experiments). (E) Immunofluorescence with Myog. (F) Box plot representation of the Myog-positive fraction among total nuclei identified by Hoechst staining. Ten different fields from images like (E) were analyzed. Scale bar, 20 μm.