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. 2014 Dec 29;43(2):917–931. doi: 10.1093/nar/gku1348

Figure 8.

Figure 8.

Energy transfer changes in the 12RSS during the SC to PC transition are sensitive to the integrity of the 23RSS heptamer and nonamer. Bars represent the average energy transfer efficiency from two independent measurements, with dots indicating each experimental value and the content of the reaction indicated below each bar. The free DNA, SC and 23RSS partner (PC) reactions were performed as with the 12RSSdR2a substrate in Figure 2B and D. Reactions were also performed with a partner 23RSS with a mutant nonamer (Mut Non) or a scrambled heptamer (ScrmHep). (A) 12RSSdE1a substrate. (B) 12RSSdJ3a substrate.