Table 4. SAXS data collection and scattering-derived parameters for EcMazE–DNA complexes.
EcMazE1–50-‘a’ | EcMazE-‘a’ | |
---|---|---|
Data collection parameters | ||
Beam line | SWING | SWING |
HPLC column | KW402.5–4F | KW402.5–4F |
Wavelength (Å) | 1.03 | 1.03 |
q range | 0.012–0.299 | 0.012–0.331 |
Injected concentrations | 90 μl at 7 mg/ml | 90 μl at 8 mg/ml |
Temperature (K) | 283 | 283 |
Structural parameters | ||
I(0) (from Guinier) | 0.52 ± 0.30.10−2 | 0.24.10−3 ± 0.38.10−6 |
Rg (Å) (from Guinier) | 25.51 ± 0.19 | 30.26 ± 0.07 |
I(0) (from p(r)) | 0.51 ± 0.10.10−2 | 0.24.10−3 ± 0.21.10−6 |
Rg (Å) (from p(r)) | 24.78 ± 0.06 | 30.96 ± 0.03 |
Dmax (Å) | 71 | 91 |
Molecular mass determination | ||
SAXSMoW (kDa)a | 29.0 | 32.3 |
QR (kDa)a | 28.1 | 30.2 |
Theoretical MM from sequence (kDa) | 24.3 | 31.7 |
Model statistics | ||
χ2 | 0.64 | 0.96 |
χ2free | 0.92 | 1.15 |
RSAS (%) | 0.9927 | 0.0126 |
aFor the q range reported above in the table.