Skip to main content
. 2015 Jan 6;43(2):1241–1256. doi: 10.1093/nar/gku1352

Table 4. SAXS data collection and scattering-derived parameters for EcMazE–DNA complexes.

EcMazE1–50-‘a’ EcMazE-‘a’
Data collection parameters
Beam line SWING SWING
HPLC column KW402.5–4F KW402.5–4F
Wavelength (Å) 1.03 1.03
q range 0.012–0.299 0.012–0.331
Injected concentrations 90 μl at 7 mg/ml 90 μl at 8 mg/ml
Temperature (K) 283 283
Structural parameters
I(0) (from Guinier) 0.52 ± 0.30.10−2 0.24.10−3 ± 0.38.10−6
Rg (Å) (from Guinier) 25.51 ± 0.19 30.26 ± 0.07
I(0) (from p(r)) 0.51 ± 0.10.10−2 0.24.10−3 ± 0.21.10−6
Rg (Å) (from p(r)) 24.78 ± 0.06 30.96 ± 0.03
Dmax (Å) 71 91
Molecular mass determination
SAXSMoW (kDa)a 29.0 32.3
QR (kDa)a 28.1 30.2
Theoretical MM from sequence (kDa) 24.3 31.7
Model statistics
χ2 0.64 0.96
χ2free 0.92 1.15
RSAS (%) 0.9927 0.0126

aFor the q range reported above in the table.