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Processivity. Primer extension assays were performed by mixing preformed enzyme–DNA (50/80-mer) complexes with magnesium acetate, physiological concentration of dNTP (21), and heparin. The reactions were stopped either after 1 or 2 min as indicated in the figure. Reactions allowing multiple binding events in the absence of heparin are indicated in the figure. The reaction products were resolved on a 10% denaturing acrylamide gel.
