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Effect of high-LET and low-LET IR on Bcl2/Mre11 and Bcl2/Ku70 binding. (A) Western blot analysis of Bcl2, Mre11, Rad50 and NBS1 in BEAS-2B cells overexpressing Bcl2 or vector-only control. (B), (C) and (D) BEAS-2B cells overexpressing Bcl2 were treated with increasing doses of 56Fe, 28Si or X-ray. The co-IP experiments were performed using agarose-conjugated Bcl2 antibody. Bcl2-associated Mre11 or Ku70 were analyzed by western blot.
