Figure 5.

Bcl2 directly interacts with Mre11 via its BH1 and BH4 domain. (A) Purified recombinant WT Bcl2 (20 ng/ml) was incubated with purified Mre11, or NBS1 or RAD50 (20 ng/ml) in 1% CHAPS lysis buffer at 4°C for 2 h. Co-IP experiments were performed using Bcl2, MRE11 or NBS1 or RAD50 antibody, respectively. Bcl2, Mre11, NBS1 and RAD50 were analyzed by western blot analysis. (B) Purified WT or each BH deletion Bcl2 mutant protein (20 ng/ml) was incubated with 20 ng/ml of purified Mre11 in 1% CHAPS lysis buffer at 4°C for 2 h. Co-IP was carried out using agarose-conjugated Bcl2 antibody. Bcl2 and Mre11 were analyzed by western blot.