Figure 2.

Circular chromosome conformation capture sequencing (4C-seq) analysis of long-range interactions at the Ptpn22 locus in a functional FOXP3 model. (A) Purified CD4+ T cells (top panel) were transduced with MSCV-NGFR (MINR1) empty vector (bottom left panel) or MINR1-FOXP3 (bottom right panel). (B) Transduced cells were subjected to gene expression analyses by qRT-PCR (top panels), and FOXP3 binding to endogenous target genes was measured using ChIP-qPCR (bottom panel). (C) Immunoregulatory function was assessed in an in vivo IBD model in which naive, pathogenic CD4+ T cells are co-transferred into immunodeficient mice together with PBS (black), empty vector-transduced cells (red) or FOXP3-transduced cells (blue). (D) Empty vector- and FOXP3-transduced cells were subjected to 4C analysis to assess genome-wide interactions at the Ptpn22 locus.