Figure 4.

In vitro cleavage activity of Tbis1, Sco5333 and Sco5333_C252D to methylated plasmid DNA is suppressed by Zn²⁺. (A) 0–8 mM Zn²⁺ is added into the in vitro cleavage reaction system of Tbis1 in presence of 2 mM Mn²⁺ or Co²⁺. (B) 0–8 mM Zn²⁺ is added into the in vitro cleavage reaction system of Sco5333 in presence of 2 mM Mg²⁺ or Mn²⁺. Cleavage efficacy of Sco5333 decreases along with the increasing Zn²⁺ concentration. (C) 0–8 mM Zn²⁺ is added into the in vitro cleavage reaction system of Sco5333_C252D in presence of 2 mM Mg²⁺ or Mn²⁺. Cleavage efficacy of Sco5333_C252D decreases with the increasing Zn²⁺ concentration.