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. 2015 Jan 7;43(2):1044–1055. doi: 10.1093/nar/gku1383

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Determination of the co-transcription of the csa1, cas1, cas2 and cas4 genes. (A) Organization of csa1 (852 bp), cas1 (873 bp), cas2 (270 bp) and cas4 (528 bp) genes is depicted schematically. The locations of primers are indicated that were used for RT-PCR to determine co-transcription. The intergenic regions between csa1 and cas1 is 42 bp and between cas1 and cas2 is 110 bp; cas2 and cas4 are overlapped. (B) Fragments obtained in RT-PCR experiments carried out with the primers csa1qF and cas1qR (lane 1, product 1016 bp), csa1qF and cas2qR (lane 2), csa1qF and cas4qR (lane 3), cas1qF and cas2qR (lane 4, product 1151 bp), cas1qF and cas4qR (lane 5, product 1418 bp), and cas2qF and cas4qR (lane 6, product 376 bp). A DNA ladder (Trans 2K plus II) was run in lane L as a size marker.