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. 2015 Jan 7;43(2):1044–1055. doi: 10.1093/nar/gku1383

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Csa3a triggers CRISPR spacer acquisition in Sulfolobus. (A) Spacer acquisition was detected by PCR for both loci in csa3a overexpression strain. C1: S. islandicus E233S strain, C2: E233S carrying empty vector pSeSD, OEcsa3a: E233S carrying csa3a overexpression plasmid. The csa3a expression plasmid is shown with individual genes indicated (B and C). Locations of the protospacers corresponding to newly acquired plasmid-derived spacers on locus 1 (B) and locus 2 (C) are shown in the inner (forward strand) and outer (reverse strand) circles.