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. 2015 Jan 8;43(2):1012–1018. doi: 10.1093/nar/gku1391

Figure 3.

Figure 3.

The effects of N3-CMdT and O4-CMdT on DNA transcription mediated by T7 RNAP and HeLa nuclear extract (hRNAPII) in vitro. (a) Sample processing for NcoI/SfaNI-mediated restriction digestion/postlabeling assay (P* indicates a 32P-labeled phosphate group). (b) Representative gel images showing the NcoI/SfaNI-produced restriction fragments of interest. The restriction fragment arising from the competitor vector, i.e. d(CATGGCGATATGCTAT), is designated as ‘16mer-Comp’, ‘13mer-C’, ‘13mer-A’, ‘13mer-G’ and ‘13mer-T’ represent the standard synthetic ODNs d(CATGGCGNGCTAT), where ‘N’ is C, A, G and T, respectively. (c) Sample processing for MluCI/Cac8I-mediated restriction digestion/postlabeling assay. (d) Representative gel images showing the MluCI/Cac8I-generated restriction fragments of interest. ‘10mer-C’, ‘10mer-A’, ‘10mer-G’ and ‘10mer-T’ represent the standard synthetic ODNs d(AATTATAGCM), where ‘M’ is C, A, G and T, respectively. (e) The RBE values of N3-CMdT and O4-CMdT in in vitro transcription systems. (f and g) Mutagenic properties of N3-CMdT (f) and O4-CMdT (g) in in vitro transcription systems. The data represent the mean and standard error of results from three independent experiments.