FIG 5.

The Rho GTPase Rac1 facilitates R. montanensis entry into tick cells. DVE1 cells were treated with C. difficile toxin B (ToxB), a general inhibitor for Rho GTPases (A), or with the Rac1 inhibitor (Rac) (B), and the mean (± standard error of the mean) percent relative rickettsial invasion of each treatment was compared to that of the untreated (vehicle only) control. Each concentration of inhibitor was assessed in quadruplicate, and means represent a combination of results from two independent experiments. ****, P < 0.0001.